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Peptides MEX

Peptides MEX / Glossary

Stationary phase: the retained part of a separation

A phase’s function in a separation is more informative than its physical appearance.

Updated · Sources and editorial standards

It is not always a solid

The stationary phase remains within the chromatographic system as the mobile phase moves through it. It may be a solid, a gel or a liquid retained on a support. Stationary phase and solid phase are therefore not exact synonyms.

Sources: [1]

Comparing methods in context

If reports use different columns, do not assume a compound will appear at the same time. Column description is part of the method. A resin used to assemble a peptide serves a different synthetic purpose from analytical separation.

Sources: [1]

Questions about this reference

Is every stationary phase a synthesis resin?

No. Chromatographic separation and supporting molecular construction are different roles.

[1]

Sources and original reading

  1. IUPAC Gold Book: stationary phase in chromatography

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