Peptides MEX / Glossary
Mobile phase: fluid, eluent and method conditions
In a chromatographic description, identify which fluid moves through the system and how it changes during analysis.
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The moving phase
The mobile phase is the fluid that passes through or along the stationary phase. In liquid chromatography it is liquid; other methods may use gas or supercritical fluid. Eluent is a term for the mobile phase in elution chromatography.
Sources: [1]
What to retain from a report
Mobile-phase composition forms part of the procedure. It need not match the solvent used to prepare the sample. If a report specifies a gradient, record that composition changes during separation before comparing retention times.
Sources: [1]
Questions about this reference
Does mobile phase always mean water?
No. The term describes a role in the system; composition depends on the method.
Sources and original reading
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