Peptides MEX / Glossary
Retention time: measuring and comparing a peak
Retention time belongs to a component under the conditions of a particular separation.
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From sample introduction to the peak maximum
Total retention time runs from sample introduction to the appearance of the component’s peak maximum. It includes the passage time of an unretained species. It is not necessarily the duration of the entire chromatographic run.
Total versus adjusted time
Adjusted retention time subtracts the passage time of an unretained species. Values from tables using different conventions are not directly comparable. Retain the method as well: a time match without context does not provide complete identification by itself.
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